What are the characteristics of this type of inhibitor?

The inhibition of the alcohol dehydrogenase by a formamide compo Show more Inhibition of Alcohol Dehydrogenase The inhibition of the alcohol dehydrogenase by a formamide compound is examined. Alcohol dehydrogenase (ADH) is the enzyme that is responsible for converting ethanol to acetaldehyde. It is the enzyme responsible for the metabolism of ethanol in the beverages we consume. 5 different isoenzymes of ADH have been identified and it has been shown that the enzyme has rather a broad substrate specificity and can oxidize aldehydes as well as primary and secondary alcohols. For example ADH can also oxidize methanol (wood alcohol) and ethylene alcohol (antifreeze). The poisonous nature of these compounds results from the ADH-catalyzed conversion of these compounds to toxic products. For example ADH converts methanol to formaldehyde which is toxic to the optic nerve and can produce blindness. In high doses formaldehyde may be fatal. In this study the authors investigated the ability of formaldehyde compounds to inhibit alcohol dehydrogenase. Only a portion of their data is presented here. The authors were able to propose a mechanism for the inhibition from the extensive data they collected using a wide variety of formamide compounds. The authors of this study studied the ability of N-15-dimethylhexylformamide to inhibit mouse ADH1. The activity of the enzyme was measured in the absence of inhibitor and in the presence of 1.0 M inhibitor. The data are presented in the table below. KM of ADH1 w/o inhibitor = 0.3069 & Vmax of ADH1 w/o inhibitor = 0.0934 KM of ADH1 w/ inhibitor = 0.1157 & Vmax of ADH1 w/ inhibitor = 0.035 Calculate the value of . if its significantly different from 1. What kind of inhibitor is N-15-dimethylhexylformamide? Explain using the data provided above. What are the characteristics of this type of inhibitor? Calculate the KI for N-15-dimethylhexylformamide. PLEASE MAKE SURE TO FULLY EXPLAIN YOUR ANSWER. ANY REFERENCES YOU USE MUST BE CITED. Show less

QUICK QUOTE

Approximately 250 words